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| Name | Goat anti Mouse IgM mu chain FITC |
|---|---|
| Catalog number | 43R-1370 |
| Size | 2 mg |
| Price | € 352.00 |
| Category | Secondary Antibody |
|---|---|
| Antibody Subtype | Conjugated Secondary Antibodies |
| Area of research | Immunology |
| Type of Immunogen | Purified Mouse IgM, u chain |
| Raised in | Goat |
| Specificity | This antibody reacts with heavy (mu) chains on mouse IgM. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin mouse serum proteins or light chains on all mouse immunoglobulins or human IgG or serum proteins. |
| Conjugate/Tag | FITC |
| Grade & Purity | Grater than 95% pure |
| Method of Purification | Affinity purified using solid phase Mouse IgM |
| Source | Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Concentration | 1.0 mg/ml (lot specific) |
| Form & Buffer | Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. |
| Storage | Store at 2-8 deg C. |
| Shipping conditions | Blue Ice |
| Tested for | FC; IF; IHC |
| Usage Recommendations | The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody. |
| Properties | This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen |
| Conjugation | Anti-FITC Antibody |
| Latin name | Capra aegagrus hircus,Mus musculus |
| Additional isotype | IgM |
| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |