Goat anti Mouse IgG H + L FITC

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Main information

NameGoat anti Mouse IgG H + L FITC
Catalog number43R-1347
Size500 µg
Price€ 167.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenPurified Mouse IgG (H&L)
Raised inGoat
SpecificityThis antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.
Cross ReactivityNo reactivity is observed to non-immunoglobulin mouse serum proteins.
Conjugate/TagFITC
Grade & PurityGrater than 90% pure
Method of PurificationAffinity purified using solid phase Mouse IgG (H&L)
SourceGoat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Concentration1.0 mg/ml (lot specific)
Form & BufferClear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
StorageStore at 2-8 deg C.
Shipping conditionsBlue Ice
Tested forFC; IF; IHC
Usage RecommendationsThe optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
ConjugationAnti-FITC Antibody
Latin nameCapra aegagrus hircus,Mus musculus
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotypeIgG
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.