Goat anti Mouse IgG H + L biotin

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Main information

NameGoat anti Mouse IgG H + L biotin
Catalog number43R-1328
Size2 mg
Price€ 253.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenPurified Mouse IgG (H&L)
Raised inGoat
SpecificityThis antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.
Cross ReactivityNo reactivity is observed to non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, Pig, or rabbit.
Conjugate/TagBiotin
Grade & PurityGrater than 95% pure
Method of PurificationAffinity purified using solid phase Mouse IgG (H&L)
SourceGoat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Concentration1.0 mg/ml (lot specific)
Form & BufferClear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
StorageStore at 2-8 deg C.
Shipping conditionsBlue Ice
Tested forPlease refer to the datasheet
Usage Recommendations1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
ConjugationBiotinylated
Latin nameCapra aegagrus hircus,Mus musculus
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotypeIgG
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.