Goat anti Mouse IgG + IgM H + L biotin

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Main information

NameGoat anti Mouse IgG + IgM H + L biotin
Catalog number43C-CB5173
Size1 mg
Price€ 327.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenGoat anti-mouse IgG/IgM (H+L) (biotin) was raised in goat using Mouse IgG and IgM whole molecules ..
Raised inGoat
SpecificityMurine IgG/IgM
Cross ReactivityShould be determined by the end user
Conjugate/TagBiotin
Method of PurificationGoat anti Mouse IgG + IgM (H + L) (biotin) was purified by affinity chromatography.
Form & BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditionsBlue Ice
Tested forELISA; IHC; WB
Usage RecommendationsELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
ConjugationBiotinylated
Latin nameCapra aegagrus hircus,Mus musculus
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotypeIgG,IgM
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.