Goat anti Mouse IgG + IgM H + L HRP

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Main information

NameGoat anti Mouse IgG + IgM H + L HRP
Catalog number43C-CB5174
Size1 mg
Price€ 414.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenGoat anti-mouse IgG/IgM (H+L) (HRP) was raised in goat using murine IgG and IgM whole molecules ..
Raised inGoat
SpecificityMurine IgG/IgM
Cross ReactivityShould be determined by the end user
Conjugate/TagHRP
Method of PurificationGoat anti Mouse IgG + IgM (H + L) (HRP) was purified by affinity chromatography.
Form & BufferLyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-I mMunoglobulin and Protease free. 0.01% (w/v) Gentamicin Sulfate (Do NOT add NaN3!)
StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditionsBlue Ice
Tested forELISA; IHC; WB
Usage RecommendationsELISA: 1:40,000, IHC: 1:500-1:2,500, WB: 1:2,000-1:10,000
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Latin nameCapra aegagrus hircus,Mus musculus
PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
ConjugationHRP Conjugated
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotypeIgG,IgM
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.