Goat anti Mouse IgG2b FITC

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Main information

NameGoat anti Mouse IgG2b FITC
Catalog number43R-IG066ft
Size1 mg
Price€ 290.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Raised inGoat
SpecificityMurine
Cross ReactivityShould be determined by the end user
Conjugate/TagFITC
Method of PurificationGoat anti Mouse IgG2b (FITC)
Concentration1.0 mg/ml
Form & BufferPBS pH 7.2, with 0.2% BSA and 0.1% NaN3.
StorageStore at 4 deg C. DO NOT FREEZE.
Shipping conditionsBlue Ice
Tested forICC; IHC
Usage RecommendationsICC: 1:200-1:2,000, IHC: 1:200-1:2,000
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
ConjugationAnti-FITC Antibody
Latin nameCapra aegagrus hircus,Mus musculus
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.