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| Name | Goat anti Mouse IgM biotin |
|---|---|
| Catalog number | 43C-CB5117 |
| Size | 2 mg |
| Price | € 414.00 |
| Category | Secondary Antibody |
|---|---|
| Antibody Subtype | Conjugated Secondary Antibodies |
| Area of research | Immunology |
| Type of Immunogen | Goat anti-mouse IgM (biotin) was raised in goat using murine IgM heavy chain .. |
| Raised in | Goat |
| Specificity | Murine IgM |
| Cross Reactivity | Should be determined by the end user |
| Conjugate/Tag | Biotin |
| Method of Purification | Goat anti Mouse IgM (biotin) was purified by affinity chromatography. |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free. |
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Blue Ice |
| Tested for | ELISA; IHC; WB |
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody. |
| Properties | Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen |
| Conjugation | Biotinylated |
| Latin name | Capra aegagrus hircus,Mus musculus |
| Additional isotype | IgM |
| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |