Mouse anti Human IgE

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Main information

NameMouse anti Human IgE
Catalog number10-I104B
Size1 mg
Price€ 369.00


More details

CategorySecondary Antibody
Antibody SubtypeUnconjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenHuman IgE antibody was produced from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgE from myelomatous human serum
Raised inMouse
SpecificityHuman IgE CE3 domain specific
Cross ReactivityNo cross reactivity to IgG, IgM, IgA or IgD
IsotypesIgG1
CloneM12619E2
Method of PurificationHuman IgE antibody was purified by chromatography on protein A Sepharose.
SourceProduced in vitro
Form & BufferPBS, pH 7.4, 0.1 % NaN3.
StorageStore at 4 deg C.
Shipping conditionsBlue Ice
Tested forELISA
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
Additional isotypeIgE
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus