Mouse anti Rat IgD FITC

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Main information

NameMouse anti Rat IgD FITC
Catalog number61R-I149aFT
Size1 mg
Price€ 830.00


More details

CategorySecondary Antibody
Antibody SubtypeConjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenMouse anti-rat IgD (FITC) was raised in mouse using rat IgD ..
Raised inMouse
SpecificityRat
Cross ReactivityShould be determined by the end user
IsotypesIgG1 kappa
CloneMARD3
Conjugate/TagFITC
Concentration1.0 mg/ml
Form & BufferPBS with 0.1% NaN3 and 50% glycerol.
StorageAliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
Shipping conditionsBlue Ice
Tested forELISA; IF; IHC-F; IP; WB
Usage RecommendationsOptimal conditions to be determined by end user
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of mature B-lymphocytes where it is usually coexpressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum for virus detection. Anti-viral immunoglobulin D antibodies have a diagnostic value.
ConjugationAnti-FITC Antibody
Additional isotypeIgD
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus,Rattus norvegicus
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.