Mouse anti Rat IgG Light Chain HRP

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Main information

NameMouse anti Rat IgG Light Chain HRP
Catalog number40R-1002
Size100 µg
Price€ 290.00


More details

CategorySecondary Antibody
Antibody SubtypeUnconjugated Secondary Antibodies
Area of researchImmunology
Type of ImmunogenIgG Light Chain antibodies were raised in Mouse using Rat IgG ..
Raised inMouse
SpecificityRat
IsotypesIgG2b
CloneKT100
Method of PurificationMouse anti Rat IgG Light Chain antibody was purified by Protein A chromatography.
Concentration1 mg/ml
Form & BufferProvided in PBS containing 0.1% NaN3 as a preservative
StorageStore at 4 deg C for up to 1 month. For long term storage, aliquot and store at -20 to -80 deg C. Avoid repeated freeze/thaw cycles.
Shipping conditionsBlue Ice
Tested forELISA; WB
Usage RecommendationsELISA: 1:3000; WB: 1:1000-1:10000
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
ConjugationHRP Conjugated
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Additional isotypeIgG
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus,Rattus norvegicus