Mouse anti CD4

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Main information

NameMouse anti CD4
Catalog number0041
Size100 μg
Price€ 195.00


More details

CategoryPrimary Antibodies
Long descriptionIdentification of human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.
Antibody come fromDerived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone7E14
Antigen-antibody binding interactionMouse anti CD4 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
Research interestCD Marker
ApplicationFlow Cytometry
Antibody's suited forPBMC: Add10 µl of MAB/10 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
Storage-20ºC
Relevant references1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans,R.L., Wall,D.W., Platsoucas,C.D., Siegal,F.P., Fikrig,S.M., Testa,C.M, and Good,R.A. Proc. Nat. Acad. Sci. 78,544,1981. _x000B__x000B_2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement,L.T., Vink,P.E., Bradley,G.E. J. Immunology 145(1):102-8,1990 . _x000B__x000B_3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo,G., Miller,C.L., Kodys,K., J. Leukoc. Biol. 47(2): 111-20,1990. _x000B__x000B_4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond,D.C., Finberg,R., Chaudhuri,S., Sleckman,B.P., Burakoff,S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990. _x000B__x000B_5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins,B., J. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus _x000B__x000B_6. Lusso,P., De Maria,A., Malnati,M., Lori,F., DeRocco,S.E., Baseler,M., Gallo,R.C., Nat. 349(6309):533-5,1991. _x000B__x000B_7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. _x000B__x000B_8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. _x000B__x000B_Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486._x000B__x000B_Product Specific References: _x000B__x000B_1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus