Mouse anti CD15

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Main information

NameMouse anti CD15
Catalog number0151
Size100 μg
Price€ 195.00


More details

CategoryPrimary Antibodies
Long descriptionIdentification of CD15 that recognizes a human mylelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.1. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils. It is present at low density on circulating monocytes. In lymphoid tissue CD15 reacts with Reed-Sternberg cells of Hodgkins disease and with granulocytes. However, CD15 reacts with only a few tissue macrophages and does not react with dendritic cells.
Antibody come fromn/a
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneARE
Antigen-antibody binding interactionMouse anti CD15 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
Research interestCD Marker
ApplicationFlow Cytometry
Antibody's suited forPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage-20ºC
Relevant references1. Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dšrken B, Gilks WR, et al, eds. LeucocyteTyping IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989;800-805. _x000B__x000B_2. Hanjan SNS, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopath. 1982;23:172. _x000B__x000B_3. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984;82:29. _x000B__x000B_4. Pinkus GS, Thomas P, Said JW. Leu-M1ÑA marker for Reed Sternberg cells in Hodgkin's Disease: An immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus