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| Name | Mouse anti CD25 |
|---|---|
| Catalog number | 0251 |
| Size | 100 μg |
| Price | € 195.00 |
| Category | Primary Antibodies |
|---|---|
| Long description | Identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen. |
| Antibody come from | CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes. |
| Other description | Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography |
| Clone | ITYV |
| Antigen-antibody binding interaction | Mouse anti CD25 Antibody |
| Antibody is raised in | Mouse |
| Antibody's reacts with | Human |
| Antibody's reacts with these species | This antibody doesn't cross react with other species |
| Antibody's specificity | No Data Available |
| Research interest | CD Marker |
| Application | Flow Cytometry |
| Antibody's suited for | PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| Storage | -20ºC |
| Relevant references | 1. A Monoclonal Antibody (Anti-Tac) Reactive with Activated and Functionally Mature Human T Cells. Uchiyama T., Broder S., Waldmann TA, 1981 J. Immunol.126,1393. _x000B__x000B_2. Direct Demonstration of the Identity of T Cell Growth Factor Binding Protein and the Tac Antigen. Robb RJ, Greene WC, J. Exp. Med. 1983,158;1332. _x000B__x000B_3. Patients with HIV infection have a reduced proportion of lymphocytes expressing the IL2 receptor p55 chain (TAC, CD25). Zola H., Koh L.Y., Mantzioris B.X., Rhodes D., Clin. Immunol. Immunopathol. 1991 Ap:59(1):16-25. _x000B__x000B_4. Spontaneous lymphocyte proliferation in HTLV-I/II infection reflects preferential activation of CD8 and CD16/56 cell subsets. Prince H.E., Weber D.M., Jensen E.R., Clin. Immunol. Immunopathol. 1991, May;58(3):419-30. _x000B__x000B_5. Defective clonogenic potential of CD8+ T lymphocytes in patients with AIDS. Expansion in vivo of a nonclonogenic CD3+ CD8+ DR+ CD25- T cell population. Pantaleo G., Keonig S., Baseler M., Lane H.C., Fauci A.S., J. Immunol. 1990 Mar ; 144(5):1696-704. |
| Protein number | see ncbi |
| Warnings | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
| Latin name | Mus musculus |