Mouse anti CD27

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Main information

NameMouse anti CD27
Catalog number0271
Size100 μg
Price€ 195.00

More details

CategoryPrimary Antibodies
Long descriptionThe CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70.
Antibody come fromn/a
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Antigen-antibody binding interactionMouse anti CD27 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
Research interestCD Marker
ApplicationFlow Cytometry
Antibody's suited forPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Relevant references1. Morimoto C., Cluster report : CD27. In Schlossman SF., Boumsell L., Gilks W., et al., eds. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press: 1995:356-357. _x000B__x000B_2. CD27 induction on thymocytes. J Immunol 1990 Sep ;145(5):1356-63 Martorell J; Rojo I; Vilella R; Martinez-Caceres E; Vives J _x000B__x000B_3. CD27 expression by a distinct subpopulation of human B lymphocytes. Eur J Immunol 1990 De;20(12):2679-84 Maurer D; Holter W; Majdic O; Fischer GF; Knapp W _x000B__x000B_4. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol 1991 Apr 1;146(8):2488-94 de Jong R; Loenen WA; Brouwer M; van Emmerik L; de Vries EF; Borst J; van Lier RA _x000B__x000B_5. The T-cell activation antigen CD27 is a member of the growth factor/tumor necrosis factor receptor gene family. Camerini D., Walz G., Loenen W., Borst J., Seed B. J. Immunol. 1991:147(9):3165-3169
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus