Mouse anti CD117

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Main information

NameMouse anti CD117
Catalog number1171
Size100 μg
Price€ 426.00


More details

CategoryPrimary Antibodies
Long descriptionCD117 reacts with 8% of myelomonocytes and 75% of CD34-positive cells. The hematopoietic stem cells are included in this fraction and therefore, this gene product is considered to have an important function for differentiation and proliferation of hematopoietic cells. CD117 also reacts with placenta, brain, a part of acute myeloblastic leukemia cells and chronic myeloblastic leukemia cells, but not with acute lymphoblastic leukemia cells. MW is 145kDa.
Antibody come fromDerived from hybridization of mouse myeloma cells and spleen cellsof a BALB/c mouse, which was immunized with transfected cells with c-kit DNA in human leukemia cells (UT-7).
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneNU-c-kit
Antigen-antibody binding interactionMouse anti CD117 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
Research interestCD Marker
ApplicationFlow Cytometry
Antibody's suited forPBMC: Count the number of positive cells in peripheral blood. Add 10 µl of Mab to 50 µl suspended lymphocyte solution at 2 x 10^7. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage-20ºC
Relevant references1. Escribano L, Ocqueteau M, Almeida J, Orfao A, San Miguel JF. Leuk. Lymphoma (1998, in press). _x000B__x000B_2. Ocqueteau M, Orfao A, Garc’a-Sanz R, Almeida J, Gonz‡lez M, San Miguel JF. Br. J. Haematol. 95: 489-93_x000B_(1996). _x000B__x000B_3. Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, vanÕt Veer MB. Leukemia 9: 1783-86 (1995). _x000B__x000B_4. Escribano L, Orfao A, Blood (1998, in press) _x000B__x000B_5. Escribano L, Orfao A, Villarrubia J, Mart’n F, Madruga JI, Cuevas M, Velasco JL, Rios A, San Miguel JF._x000B_Cytometry 30: 98-102 (1997) _x000B__x000B_6. Macedo A, Orfao A, Mart’nez A, Vidriales MB, Valverde B, L—pez-Berges MC, San Miguel JF. Br. J. Haematol._x000B_89: 338-41 (1995).
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus