Mouse anti E1A (adenovirus early region 1)

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Main information

NameMouse anti E1A (adenovirus early region 1)
Catalog numberA200M
Size100 μg
Price€ 304.00


More details

CategoryPrimary Antibodies
Long descriptionThe immunogen for the anti- 2 E1A was full length recombinant adenovirus 2 E1A protein. The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif
Antibody come fromFull length recombinant adenovirus 2 E1A protein
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneM73
Antigen-antibody binding interactionMouse anti E1A (adenovirus early region 1) Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationImmunoprecipitation, Immunofluorescense
Antibody's suited forApplications: Immunoprecipitation (1-2 ug/ml) and 2-5 ug/ml for Immunofluorescence.
Storage-20ºC
Relevant references1) Harlow E. et. al. (1986) Association of adenovirus early region 1A proteins with cellular peptides) Mol. Cell Biol., 6 1579-1589 _x000B__x000B_2) Harlow, E. et. al. (1985) Monoclonal antibodies specific for adenovirus E1A proteins: extensive heterogeneity in the E1A products J. Virology 3: 533-546
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Tissueregion
Virusadenovirus
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus