| Category | Primary Antibodies |
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| Long description | Endothelin cell differentation gene-3 (EDG-3) belongs to a family of G-protein couple receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family. They have been implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-3 is expressed in cardiovascular, leukocyte-containing and other tissues. |
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| Antibody come from | Human Endothelial Differentiation Gene-3 unique C-terminal peptide |
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| Other description | Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography |
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| Clone | AS61 |
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| Antigen-antibody binding interaction | Mouse anti Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT Antibody |
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| Antibody is raised in | Mouse |
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| Antibody's reacts with | Human, Rat |
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| Antibody's reacts with these species | This antibody doesn't cross react with other species |
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| Antibody's specificity | No Data Available |
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| Application | Western Blot |
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| Antibody's suited for | This antibody can be used for Western blot at 1 to 5 µg/ml. Can be used to visualize EDG-3 expressed as an approximately 45 kDa protein in human and rat spleen and lung tissue (5 microgram protein sample). Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. PierceÕs SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions. |
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| Storage | -20ºC |
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| Relevant references | 1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998, 12, 1589-98._x000B__x000B_2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998, 30-31, 147-57. |
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| Protein number | see ncbi |
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| Warnings | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
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| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels. |
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| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
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| Latin name | Mus musculus |
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