Goat anti Mouse IgG1 (subclass specific)

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Main information

NameGoat anti Mouse IgG1 (subclass specific)
Catalog numberGAM/IgG1
Size1 mL
Price€ 209.00

More details

CategorySecondary Antibodies
Long descriptionThe reactivity of the antiserum is directed to the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody come fromPools of purified homogenous IgG1 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Antigen-antibody binding interactionGoat anti Mouse IgG1 (subclass specific) Antibody
Antibody is raised inGoat
Antibody's reacts withMouse
Antibody's reacts with these speciesThis antiSerum is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity has not been tested in detail, however in double radial diffusion a reaction with Rat ha been observed.
Antibody's specificityPrecipitating polyclonal Goat antiSerum to Mouse IgG1, subclass specific
Research interestVeterinary
Antibody's suited forImmunoprecipitation. Precipitin titre not les than 1:32 when tested against pooled normal mouse serum in agar block immunodiffusion titration.
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Latin nameCapra aegagrus hircus,Mus musculus
Additional isotypeIgG1
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.