Goat anti Mouse major urinary protein

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Main information

NameGoat anti Mouse major urinary protein
Catalog numberGAM/MUP
Size1 mL
Price€ 209.00


More details

CategoryPrimary Antibodies
Long descriptionThe reactivity of the antiserum is restricted to mouse MUP. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse plasma characteristic precipitin lines are obtained which show a reaction of identity with the precipitin lines obtained with purified immunogen. No reaction is obtained with any other plasma protein components or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify or measure the concentration of MUP. Proteins isolated from urine, such as Bence Jones proteins, may contain MUP as an impurity. This antiserum can be used to identify the impurity and also to remove it by immunoaffinity adsorption. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included.
Antibody come fromThe MUP is a sex related complex with a pre-albumin mobility in gel electrophoresis. Concentrations in male mice are between 50 to 150 μg/ml, in female mice 10 to 20 μg/ml. The complex is also detectable in serum, however concentrations are about 200 to 400 times lower. Variations in the composition of the complex are genetically determined. Purified mouse major urinary proteins are isolated from pooled normal urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
ClonePolyclonal
Antigen-antibody binding interactionGoat anti Mouse major urinary protein Antibody
Antibody is raised inGoat
Antibody's reacts withMouse
Antibody's reacts with these speciesThe antiSerum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Antibody's specificityPrecipitating polyclonal Goat antiSerum to Mouse major urinary proteins.
Research interestVeterinary
ApplicationImmunoprecipitation
Antibody's suited forImmunoprecipitation. In immunoelectrophoresis use 2 μl urine, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre 1:32 when tested against pooled normal mouse urine in agar-block immunodiffusion titration.
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Latin nameCapra aegagrus hircus,Mus musculus
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.