Goat anti Mouse Serum proteins

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Main information

NameGoat anti Mouse Serum proteins
Catalog numberGAM/TSP
Size1 mL
Price€ 148.00


More details

CategoryPrimary Antibodies
Long descriptionIn immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 13 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
Antibody come fromPooled whole mouse serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
ClonePolyclonal
Antigen-antibody binding interactionGoat anti Mouse Serum proteins Antibody
Antibody is raised inGoat
Antibody's reacts withMouse
Antibody's reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.
Antibody's specificityPrecipitating polyclonal Goat antiSerum to Mouse Serum proteins.
Research interestVeterinary
ApplicationImmunoprecipitation
Antibody's suited forImmunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Tissueserum
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Latin nameCapra aegagrus hircus,Mus musculus
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
GroupSera
AboutSerum should be stored at +5°C