| Category | Primary Antibodies |
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| Long description | Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. _x000B_IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation. |
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| Antibody come from | human recombinant interleukin 8 _x000B_ |
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| Other description | Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography |
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| Clone | AS14 |
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| Antigen-antibody binding interaction | Mouse anti Interleukin-8 (IL-8)* Antibody |
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| Antibody is raised in | Mouse |
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| Antibody's reacts with | Human |
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| Antibody's reacts with these species | This antibody doesn't cross react with other species |
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| Antibody's specificity | No Data Available |
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| Application | Enzyme Immunoassay, Immunoprecipitation, _x000B_ |
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| Antibody's suited for | Optimal concentration should be evaluated by serial dilutions.This antibody has been used as a coating antibody at 10 µg/ml in a sandwich ELISA. This antibody has been used to immunoprecipitate> 90% hIL-8 in solution _x000B_using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). Applications: Enzyme Immunoassay, Immunoprecipitation. |
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| Storage | -20ºC |
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| Relevant references | no information yet |
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| Protein number | see ncbi |
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| Warnings | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
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| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
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| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
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| Latin name | Mus musculus |
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