Mouse anti TRITC

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Main information

NameMouse anti TRITC
Catalog numberMA/TRITC
Size0,5 mg
Price€ 330.00


More details

CategoryPrimary Antibodies
Long descriptionThe reactivity of the antiserum is directed to TRITC as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:510 to 1:40; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
Antibody come fromHighly purified tetramethylrhodamine isothiocyanate isomer R.
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1.0 mg/ml. No foreign proteins added.
CloneNI 308
Antigen-antibody binding interactionMouse anti TRITC Antibody
Antibody is raised inMouse
Antibody's reacts withNot applicable
Antibody's reacts with these speciesThis antiSerum has not been tested for cross-reactivity with other fluorescent compounds.
Antibody's specificityMonoclonal Mouse antibody to tetramethylrhodamine isothiocyanate isomer 1
Research interestEpitope Tags
ApplicationIndirect immunofluorescence,ELISA
Antibody's suited forIndirect immunofluorescence,ELISA.
StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus