Mouse anti Human Bence Jones kappa (surface and hidden determinants)

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Main information

NameMouse anti Human Bence Jones kappa (surface and hidden determinants)
Catalog numberMAHu/BJK/MAb
Size0,2 mg
Price€ 400.00


More details

CategorySecondary Antibodies
Long descriptionThe reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Antibody come fromHighly purified Bence Jones kappa proteins isolated from pooled human urine.
Other descriptionPurified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. No foreign proteins added.
CloneNI 285, NI 250
Antigen-antibody binding interactionMouse anti Human Bence Jones kappa (surface and hidden determinants) Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThe antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
Antibody's specificityPurified monoclonal Mouse antibody to Human Ig kappa chain, free and bound
Research interestImmunology
ApplicationIndirect immunofluorescence,ELISA
Antibody's suited forIndirect immunofluorescence,ELISA.
StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus