| Category | Secondary Antibodies |
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| Long description | The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgD and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only. |
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| Antibody come from | Highly purified monoclonal IgD isolated from human serum |
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| Other description | Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added. |
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| Clone | NI 158 |
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| Antigen-antibody binding interaction | Mouse anti Human IgD (class specific) Antibody |
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| Antibody is raised in | Mouse |
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| Antibody's reacts with | Human |
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| Antibody's reacts with these species | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. |
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| Antibody's specificity | Monoclonal Mouse antibody to Human IgD, isotype specific |
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| Research interest | Immunology |
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| Application | Indirect immunofluorescence,ELISA |
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| Antibody's suited for | Indirect immunofluorescence,ELISA. |
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| Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days |
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| Relevant references | no information yet |
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| Protein number | see ncbi |
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| Warnings | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
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| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. |
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| Properties | Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of mature B-lymphocytes where it is usually coexpressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum for virus detection. Anti-viral immunoglobulin D antibodies have a diagnostic value. |
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| Additional isotype | IgD |
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| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
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| Latin name | Mus musculus |
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