Mouse anti Human IgG1 IgG2 IgG3 IgG4 (subclass specific)

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Main information

NameMouse anti Human IgG1 IgG2 IgG3 IgG4 (subclass specific)
Catalog numberMAHu/IgG1-4 set
Size5x0,5 mg
Price€ 749.00


More details

CategorySecondary Antibodies
Long descriptionThe reactivity of the antibody is restricted to a subclass specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting 1:2,000 to 1:10,000. These data should be interpreted as general recommendations only.
Antibody come fromHighly purified monoclonal IgG subclasses isolated from pooled human serum.
Other descriptionDelipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added.
CloneMixed
Antigen-antibody binding interactionMouse anti Human IgG1 IgG2 IgG3 IgG4 (subclass specific) Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThe antibodies do not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species.
Antibody's specificityMonoclonal Mouse ascites containing antibodies to Human IgG1, IgG2 IgG3 and IgG4 subclass specific.
Research interestImmunology
ApplicationIndirect immunofluorescence,ELISA
Antibody's suited forIndirect immunofluorescence,ELISA.
StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days Monoclonal antibodies should not be stored at a temperature below -25ºC due to the aggregation effect of the protein.
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional isotypeIgG1,IgG3
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus