Mouse anti Cytokeratin 10 / Keratin K10

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Main information

NameMouse anti Cytokeratin 10 / Keratin K10
Catalog numberMUB0319S
Size1 mL
Price€ 211.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come fromRKSE60 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with Cytokeratins from the Human epidermis.
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
CloneRKSE60
Antigen-antibody binding interactionMouse anti Cytokeratin 10 / Keratin K10 Antibody
Antibody is raised inMouse
Antibody's reacts withCanine,Human,Mouse,Rat,Swine,Zebrafish
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityRKSE60 reacts exclusively with Cytokeratin 10 which is present in Keratinizing stRatified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
Research interestCytoskeleton
ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
Antibody's suited forRKSE60 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesRamaekers, F. C., Puts, J. J., Moesker, O., Kant, A., Huysmans, A., Haag, D., Jap, P. H., Herman, C. J., and Vooijs, G. P. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview, Histochem J 15, 691-713. Puts, J. J., Moesker, O., Kenemans, P., Vooijs, G. P., and Ramaekers, F. C. (1985). Expression of Cytokeratins in early neoplastic epithelial lesions of the uterine cervix, Int J Gynecol Pathol 4, 300-13. Broers, J. L., Carney, D. N., Klein Rot, M., Schaart, G., Lane, E. B., Vooijs, G. P., and Ramaekers, F. C. (1986). Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies, J Cell Sci 83, 37-60. Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of Keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45. Bijman, J.T., Wagener, D.J.T., van Rennes, H., Wessels, J.M.C., Ramaekers, F.C.S. and van den Broek, P. (1987). Modulation of placental alkaline phosphatase activity and Cytokeratins in Human HN-1 cells by butyRate, retinoic acid, Catecholamines and histamine, Br. J. Cancer 56, 127-32. Van Erp, P.E.J., Rijzewijk, J.J., Boezeman, J.B.M., Leenders, J., de Mare, S., Schalkwijk, J., van de Kerkhof, P.C.M., Ramaekers, F.C.S. and Bauer, F.W. (1989). Flow Cytometric analysis of epidermal subpopulations from normal and psoriatic skin using monoclonal antibodies anti intermediate filaments, Am J Pathol 135, 865-70. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of Cytokeratin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. Franssen, M.E.J., Boezeman, J.B.M., van de Kerkhof, P.C.M. and van Erp, P.E.J. (2003). Monitoring hyperprolifeRative disorders in Human skin: flow cytometry of changing Cytokeratin expression, Cytometry Part B (Clinical Cytometry) 57B, 32-39.
Protein numberP13645
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus