Mouse anti Cytokeratin 13 / Keratin K13

Here you can find detailed information about selected products. Contact with us if you have nay questions to product or order proces.


Main information

NameMouse anti Cytokeratin 13 / Keratin K13
Catalog numberMUB0322S
Size1 mL
Price€ 211.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come from1C7 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
Clone1C7
Antigen-antibody binding interactionMouse anti Cytokeratin 13 / Keratin K13 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman,Zebrafish
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificity1C7 reacts exclusively with Cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia.
Research interestCytoskeleton
ApplicationImmunohistochemistry (frozen),Western blotting
Antibody's suited for1C7 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:20 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesvan Muijen, G. N., Ruiter, D. J., Franke, W. W., Achtstatter, T., Haasnoot, W. H., Ponec, M., and Warnaar, S. O. (1986). Cell type heterogeneity of Cytokeratin expression in complex epithelia and carcinomas as demonstRated by monoclonal antibodies specific for Cytokeratins nos. 4 and 13, Exp Cell Res 162, 97-113. Weikel, W., Wagner, R., and Moll, R. (1987). Characterization of subcolumnar reserve cells and other epithelia of Human uterine cervix. DemonstRation of diverse Cytokeratin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110.Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.van Niekerk, C. C., Boerman, O. C., Ramaekers, F. C., and Poels, L. G. (1991). Marker profile of different phases in the transition of normal Human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455-63.Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.Van Niekerk, C. C., Ramaekers, F. C., Hanselaar, A. G., Aldeweireldt, J., and Poels, L. G. (1993). Changes in expression of differentiation markers between normal ovarian cells and derived tumors, Am J Pathol 142, 157-77.van Dorst, E. B., van Muijen, G. N., Litvinov, S. V., and Fleuren, G. J. (1998). The limited difference between Keratin patterns of squamous cell carcinomas and adenocarcinomas is explicable by both cell lineage and state of differentiation of tumour cells, J Clin Pathol 51, 679-84.
Protein numberP13646
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus