Mouse anti Cytokeratin 14

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Main information

NameMouse anti Cytokeratin 14
Catalog numberMUB0324S
Size1 mL
Price€ 211.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come fromSPK-14 is a Mouse monoclonal IgG1, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a synthetic peptide corresponding to the carboxy-terminal sequence of Human Cytokeratin 14 (KVVSTHEQVLRTKN) coupled to keyhole limpet hemocyanin.
Other descriptionEach vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
CloneSPK-14
Antigen-antibody binding interactionMouse anti Cytokeratin 14 Antibody
Antibody is raised inMouse
Antibody's reacts withFeline,Human,Swine,Canine
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificitySPK-14 reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stRatified and combined epithelia.
Research interestCytoskeleton
ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
Antibody's suited forSPK-14 is useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration.
StorageStore at 4oC, or in small aliquots at –20oC.
Relevant referencesIvanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline Cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Bonfrer, J. M., Groeneveld, E. M., Korse, C. M., van Dalen, A., Oomen, L. C., and Ivanyi, D. (1994). Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantifiCation of tissue polypeptide antigen recognizes Keratin 18, Tumour Biol 15, 210-22.
Protein numberP02533
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus