Mouse anti Cytokeratin 18 / Keratin K18

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Main information

NameMouse anti Cytokeratin 18 / Keratin K18
Catalog numberMUB0328S
Size1 mL
Price€ 211.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come fromDE-K18 is a Mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal preparation extracted from the Human vulvar squamous carcinoma cell line A431.
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
CloneDE-K18
Antigen-antibody binding interactionMouse anti Cytokeratin 18 / Keratin K18 Antibody
Antibody is raised inMouse
Antibody's reacts withCanine,Feline,Human,Zebrafish
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityDE-K18 reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Research interestCytoskeleton
ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Antibody's suited forE-K18 is useful for immunocytochemistry, immunoblotting, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room temperature is required. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:20 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesIvanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline Cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with Keratin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of Keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61. Bonfrer, J. M., Groeneveld, E. M., Korse, C. M., van Dalen, A., Oomen, L. C., and Ivanyi, D. (1994). Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantifiCation of tissue polypeptide antigen recognizes Keratin 18, Tumour Biol 15, 210-22.
Protein numberP05783
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus