Mouse anti Cytokeratin 13

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Main information

NameMouse anti Cytokeratin 13
Catalog numberMUB0340S
Size1 mL
Price€ 211.00

More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come fromKs13.1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
Antigen-antibody binding interactionMouse anti Cytokeratin 13 Antibody
Antibody is raised inMouse
Antibody's reacts withBovine,Human,Rat
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityKs13.1 reacts exclusively with Cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia. As a result the antibody also reacts with neoplasms derived from these epithelia.
Research interestCytoskeleton
ApplicationImmunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Antibody's suited forKs13.1 is suitable for immunoblotting and immunohistochemistry on frozen tissues and paraffin embedded tissue after protease pretreatment. Optimal antibody dilution should be determined by titration.
StorageStore at 4oC, or in small aliquots at –20oC.
Relevant referencesDallenbach-Hellweg, G., Lang, G. (1991). Immunohistochemical studies on uterine tumors, Path. Res. Pract. 187, 36-43. Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W. (1987). Patterns of expression of cytoskeletal proteins in Human thyroid gland and thyroid carcinomas, Differentiation 35, 53-71. Franke, W.W., Moll, R., Achtstätter Th. and Kuhn, C. (1986) Cell typing of epithelia and carcinomas of the female genital tract using cytoskeletal proteins as markers, Banbury Report 21, Viral Etiology of Cervical Cancer, Cold Spring Harbor LaboRatory (NY), 121-148. Moll, R., Achtstätter, Th., Becht, E., Balcarova-Ständer, J., Ittensohn, M. and Franke, W.W. (1988). Cytokeratins in normal and malignant transitional epithelium, Am. J. Pathol. 132, 123-144. Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R. (1982). The Catalog of Human Cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells, Cell 31, 11-24. Demirkesen, C., Hoede, N., Moll, R. (1995). Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual Cytokeratins, J Cutan Pathol 22: 518-535.
Protein numberP13646
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus