Mouse anti Cytokeratin 8 / Keratin K8

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Main information

NameMouse anti Cytokeratin 8 / Keratin K8
Catalog numberMUB0352P
Size0,1 mg
Price€ 290.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to distinguish between different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Antibody come from35ßH11 is a Mouse monoclonal IgM antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a Balb/c Mouse immunized with a cytoskeletal preparation of the Human hepotocellular carcinoma cell line Hep3B.
Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Clone35βH11
Antigen-antibody binding interactionMouse anti Cytokeratin 8 / Keratin K8 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman,Zebrafish
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificity35ßH11 reacts exclusively with Cytokeratin 8 which is present in glandular-type epithelia and most carcinomas derived thereof. It is in general not reactive in non-epithelial tissues and cells.
Research interestCancer,Cytoskeleton
ApplicationImmunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Antibody's suited for35ßH11 is reactive on frozen tissue sections after fixation in methanol. It is particularly effective on paraffin-embedded tissues after microwave pretreatment of the slides. In addition, 35ßH11 is suitable for immunocytochemistry, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesGown, A.M., and Vogel, A.M. (1982). Monoclonal antibodies to intermediate filament proteins of Human cells: unique and cross-reacting antibodies. Journal of Cell Biology 95, 414-24.
Protein numberP05787
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus