Mouse anti Integrin beta 1D

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Main information

NameMouse anti Integrin beta 1D
Catalog numberMUB0906S
Size1 mL
Price€ 211.00

More details

CategoryPrimary Antibodies
Long descriptionIntegrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin β1: β1A and β1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. β1A is present in all tissues, except cardiac and skeletal muscle, which instead express the β1D variant.
Antibody come from2B1 is a Mouse monoclonal IgG1, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin β1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
Antigen-antibody binding interactionMouse anti Integrin beta 1D Antibody
Antibody is raised inMouse
Antibody's reacts withCanine,Human,Mouse,Swine
Antibody's reacts with these speciesA broad species reactivity is expected because of the conserved nature of the epitope.
Antibody's specificity2B1 recognizes specifically the cytoplasmic domain of integrin subunit β1D present in cardiac and skeletal muscle.
Research interestCardiovascular,Cell adhesion
ApplicationImmunocytochemistry,Immunohistochemistry (frozen),Western blotting
Antibody's suited for2B1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:20 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesvan der Flier, A., Gaspar, A. C., Thorsteinsdottir, S., Baudoin, C., Groeneveld, E., Mummery, C. L., and Sonnenberg, A. (1997). Spatial and temporal expression of the beta1D integrin during Mouse development, Dev Dyn 210, 472-86.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus