Mouse anti Reticulon-1A / NSP-A

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Main information

NameMouse anti Reticulon-1A / NSP-A
Catalog numberMUB1312S
Size1 mL
Price€ 211.00


More details

CategoryPrimary Antibodies
Long descriptionRecently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
Antibody come fromMON-162 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (β-GAL-NSP-A 6-776).
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
CloneMON162
Antigen-antibody binding interactionMouse anti Reticulon-1A / NSP-A Antibody
Antibody is raised inMouse
Antibody's reacts withHamster,Human,Mouse,Rat
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityMON-162 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-162 is loCated between amino acid residues 338-422 of Reticulon-1A.
Research interestCancer,Neurobiology
ApplicationImmunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Antibody's suited forMON-162 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, and immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:20 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesRoebroek, A.J.M., van de Velde, H.J.K., Van Bokhoven, A., Broers, J.L.V., Ramaekers, F.C.S., Van de Ven, W.J.M. (1993). Cloning and expression of alternative transcripts of a noval neuroendocrine-specific gene and identifiCation of its 135-kDa translation product. J. Biol. Chem. 268,13439-47. van de Velde, H. J., Roebroek, A. J., van Leeuwen, F. W., and Van de Ven, W. J. (1994). Molecular analysis of expression in Rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum, Mol Brain Res 23, 81-92. van de Velde, H. J., Roebroek, A. J., Senden, N. H., Ramaekers, F. C., and Van de Ven, W. J. (1994). NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum, J Cell Sci 107, 2403-16. van de Velde, H. J., Senden, N. H., Roskams, T. A., Broers, J. L., Ramaekers, F. C., Roebroek, A. J., and Van de Ven, W. J. (1994). NSP-encoded reticulons are neuroendocrine markers of a novel Category in Human lung cancer diagnosis, Cancer Res 54, 4769-76. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21 Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus