Mouse anti Neu-Oncogen (C-erb B2)

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Main information

NameMouse anti Neu-Oncogen (C-erb B2)
Catalog numberMUB1320P
Size0,1 mg
Price€ 290.00

More details

CategoryPrimary Antibodies
Long descriptionC-erbB-2 (erythroblastosis oncogene B), also known as HER2 (Human Epidermal Growth Factor Receptor 2), or Neu, CD340 and p185 is a protein that in Humans is encoded by the ERBB2 gene. AmplifiCation or overexpression of this gene has been shown to play an important role in the pathogenesis and progression of certain aggressive types of breast cancer, as well as many other epithelial malignancies and brain tumors. In recent years it has become an important biomarker and target of therapy for disease. ERBB2 is a known proto-oncogene loCated at the long arm of Human chromosome 17 (17q21-q22). The oncogene was found to code for EGFR. Gene cloning showed that HER2, Neu and ErbB-2 are all encoded by the same gene. The ErbB family is composed of plasma membrane-bound receptor tyrosine kinases, that contain an extracellular ligand binding domain, a transmembrane domain and an intracellular domain that can interact with a multitude of signaling molecules. HER2 can heterodimerise with any of the other three receptors and is considered to be the preferred dimerisation partner of the other ErbB receptors. Dimerisation results in the autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptors and initiates a variety of signaling pathways.
Antibody come from9G55 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a synthetic peptide from the C-terminus of Human c-erbB-2 protein.
Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Antigen-antibody binding interactionMouse anti Neu-Oncogen (C-erb B2) Antibody
Antibody is raised inMouse (Balb/c)
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificity9G6 Immunoprecipitates a 180 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of Human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor.
Research interestCancer
ApplicationElectron microscopy,Flow Cytometry,Immunofluorescence,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Immunoprecipitation,Western blotting
Antibody's suited for9G6 is useful for immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen and paraffin embedded tissues. Optimal antibody dilution should be determined by titration.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesVan de Vijver, M.J. Peterse, J.L. Mooi, W.J. Wisman, P. Lomans, J. Dalesio, O. and Nusse, R. (1988). Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer. N Engl J Med. 319, 1239-45.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus