Mouse anti Smoothelin

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Main information

NameMouse anti Smoothelin
Catalog numberMUB1700P
Size0,1 mg
Price€ 339.00


More details

CategoryPrimary Antibodies
Long descriptionSmoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Smoothelin has also been detected in smooth-muscle neoplasms. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is loCated on chromosome 22.
Antibody come fromR4A is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal extract of Chicken gizzard.
Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
CloneR4A
Antigen-antibody binding interactionMouse anti Smoothelin Antibody
Antibody is raised inMouse
Antibody's reacts withCanine,Chicken,Feline,Human,Monkey,Swine
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityR4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.
Research interestCardiovascular,Cytoskeleton
ApplicationImmunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting, Immunocytochemistry
Antibody's suited forR4A is suitable for immunoblotting, immunocytochemistry on permeabilised cells, and and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citRate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesvan der Loop, F. T., Schaart, G., Timmer, E. D., Ramaekers, F. C., and van Eys, G. J. (1996). Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells, J Cell Biol 134, 401-11. Wehrens, X. H., Mies, B., Gimona, M., Ramaekers, F. C., Van Eys, G. J., and Small, J. V. (1997). Localization of smoothelin in avian smooth muscle and identifiCation of a vascular-specific isoform, FEBS Lett 405, 315-20. van der Loop, F. T., Gabbiani, G., Kohnen, G., Ramaekers, F. C., and van Eys, G. J. (1997). Differentiation of smooth muscle cells in Human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype, Arterioscler Thromb Vasc Biol 17, 665-71. Christen, T., Bochaton-Piallat, M. L., Neuville, P., Rensen, S., Redard, M., van Eys, G., and Gabbiani, G. (1999). Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation, Circ Res 85, 99-107. Johansson, B., Eriksson, A., Ramaekers, F., and Thornell, L. (1999). Smoothelin in adult and developing Human arteries and myocardium, Histochem Cell Biol 112, 291-9. Johansson, B., Eriksson, A., Ramaekers, F., and Thornell, L. E. (1999). Smoothelin and intermediate filament proteins in Human aortocoronary saphenous vein by-pass grafts, Histochem J 31, 723-7. Deruiter, M. C., Rensen, S. S., Coolen, G. P., Hierck, B. P., Bergwerff, M., Debie, W. M., Gittenberger-De Groot, A. C., and Van Eys, G. J. (2001). Smoothelin expression during Chicken embryogenesis: detection of an embryonic isoform, Dev Dyn 221, 460-3. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50. Coco, D.P., Hirsch, M.S., Hornick, J.L. (2009). Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract. Am J Surg Pathol 33, 1795-1801.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus