Mouse anti VCAM-1

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Main information

NameMouse anti VCAM-1
Catalog numberMUB1905P
Size0,1 mg
Price€ 290.00

More details

CategoryPrimary Antibodies
Long descriptionVascular cell adhesion molecule-1 (VCAM-1) is induced on Human umbilical vein endothelium (HUVE) by recombinant Human tumor necrosis factor - α (rh TNF-α), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigRation at sites of inflammation and immune reaction.
Antibody come from4B9 is a Mouse IgG1 monoclonal antibody geneRated by immunization of Balb/c mice with Human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant Human (rh) TNF-α (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Antigen-antibody binding interactionMouse anti VCAM-1 Antibody
Antibody is raised inMouse (Balb/c)
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityThe antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
Research interestCancer,Immunology
ApplicationFlow Cytometry,Immunohistochemistry (frozen),Immunoprecipitation
Antibody's suited for4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant referencesCarlos. T., Schwartz. B., Kovach. N., Yee. E., Rosa. M., Osborn. L., Chi-Rosso. G., Newman. B., Lobb. R., et al. (1990). Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured Human endothelial cells Blood. 76(5) 965-70. Ockenhouse, C.F., Tegoshi, T., Maeno, Y., Benjamin, C., Ho, M., Kan, K.E., Thway, Y., Win, K., Aikawa, M. and Lobb, R.R. (1992). Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1. J Exp Med. 176, 1183-9. Bacchi, C.E., Marsh, C.L., Perkins, J.D., Carithers, R.L. Jr, McVicar, J.P., Hudkins, K.L., Benjamin, C.D., Harlan, J.M., Lobb, R. and Alpers, C.E. (1993). Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection. Am J Pathol. 142, 579-91. Pinola, M., Saksela, E., Tiisala, S. and Renkonen, R. (1994). Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation. Scand J Immunol. 39, 131-6. Chuluyan, H.E., Osborn, L., Lobb, R. and Issekutz AC. (1995). Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte trans-endothelial migRation. J Immunol. 155, 3135-4.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus