| Category | Primary Antibodies |
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| Long description | Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers. |
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| Antibody come from | 214D4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a fusion protein, Human b-gal-episialin/MUC1 repeat domain. |
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| Other description | Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide. |
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| Clone | 214D4 |
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| Antigen-antibody binding interaction | Mouse anti MUC-1 (CD227) Antibody |
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| Antibody is raised in | Mouse (Balb/c) |
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| Antibody's reacts with | Human |
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| Antibody's reacts with these species | This antibody doesn't cross react with other species |
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| Antibody's specificity | 214D4 is directed anti the repeat domain of episialin. |
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| Research interest | CD markers,Cell adhesion, Cancer |
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| Application | Flow Cytometry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin) |
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| Antibody's suited for | 214D4 is useful for flow cytometry and immunohistochemistry on frozen and paraffin embedded tissue. Optimal antibody dilution should be determined by titration. |
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| Storage | Store at 4°C, or in small aliquots at –20°C. |
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| Relevant references | Hilkens, J., et al., Tumor Biol. 19 (suppl. 1): 67-70, 1998. Wesseling, J., et al., Mol. Biol. Cell 7: 565-577, 1996. |
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| Protein number | see ncbi |
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| Warnings | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
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| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
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| Test | Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
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| Latin name | Mus musculus |
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