Mouse anti ICAM-3

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Main information

NameMouse anti ICAM-3
Catalog numberMUB2029P
Size0,1mg
Price€ 290.00


More details

CategoryPrimary Antibodies
Long descriptionIntercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a type I transmembrane glycoprotein, which normally contain 2-9 immunoglobulin-like C2-type domains, and binds to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined.
Antibody come fromMA4 is a mouse monoclonal IgG1/k antibody derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.
Other descriptionEach vial contains 100 µl 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
CloneMA4
Antigen-antibody binding interactionMouse anti ICAM-3 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityMA4 recognizes a linear epitope in domains 1 and 2 of ICAM-3. MA4 robustly inhibits apoptotic cell interaction with phagocytes
Research interestImmunology,Apoptosis
ApplicationEnzyme Linked Immuno Sorbent Assay, Flow cytometry, Immunohistochemistry on frozen sections, Immunocytochemistry, Immunoprecipitation, Western blotting.
Antibody's suited forMA4 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
StorageStore at 4°C, or in small aliquots at -20°C.
Relevant references1) Torr EE, Gardner DH, Thomas L, Goodall DM, Bielemeier A, Willetts R, Griffiths HR, Marshall LJ, Devitt A. Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells. Cell Death Differ. 2012;19: 671–679.
Protein numbersee ncbi
Warningsno dangerous / no information available then contact us to know
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus