Mouse anti HLA Class I

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Main information

NameMouse anti HLA Class I
Catalog numberMUB2035P
Price€ 290.00

More details

CategoryPrimary Antibodies
Long descriptionThe major histocompatibility complex (MHC) is a cluster of genes that play an important role in the immune response. In humans, this complex is referred to as the Human Leucocyte Antigen (HLA) system. There are three major HLA class I genes which are HLA-A, HLA-B and HLA-C. These are highly polymorphic and thousands of alleles are known to exist. The HLA-A, B and C gene products are heterodimeric cell surface glycoproteins which all consist of a 40-45kD MHC-encoded alpha chain and a non-covalently linked non-MHC encoded light chain (b2-microglobulin) of 12kDa. These proteins are found on the surface of almost all nucleated cells and their role is to present peptides, produced by intracellular protein degradation, to cytotoxic T lymphocytes and natural killer cells.
Antibody come fromBRA-23/9 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with REH cells, a human pre-B cell leukaemia cell line.
Other descriptionEach vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
Antigen-antibody binding interactionMouse anti HLA Class I Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityThe antibody BRA-23/9 is directed against a non-polymorphic determinant of human HLA Class I. The antibody has not been tested for cross reactivity with other species.
Research interestImmunology
ApplicationFlow Cytometry, Immunohistochemistry (Frozen)
Antibody's suited forThe BRA-23/9 antibody is suitable for the detection of HLA Class I antigen by flow cytometry and Immunohistochemistry on frozen tissues, for example from human tonsil, lymph nodes, as well as lymphocytes Optimal antibody dilutions for the different applications should be determined by titration.
StorageStore at 4oC, or in small aliquots at -20oC.
Relevant references1. Tanaka J, Toubai T, Tsutsumi Y, Miura Y, Kato N, Umehara S, Kahata K, Mori A, Toyoshima N, Ota S, Kobayashi T, Kobayashi M, Kasai M, Asaka M, Imamura M. (2004). Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells. Blood 104:768-74. 2. Tóth J, Kubes M. (1993). Masking of HLA class I molecules expressed on K-562 target cells can restore their susceptibility to NK cell cytolysis. Immunobiology. 188:134-44.
Protein numberUniProt: P30443 (Human)
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus