Mouse anti HLA-DR

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Main information

NameMouse anti HLA-DR
Catalog numberMUB2051P
Price€ 290.00

More details

CategoryPrimary Antibodies
Long descriptionThe major histocompatibility complex (MHC) is a cluster of genes that play an important role in the immune response. In humans, this complex is referred to as the Human Leucocyte Antigen (HLA) system and is located on chromosome 6. In humans the MHC II locus includes genes for alpha and beta subunits of the three class II MHC proteins HLA-DR, HLA-DQ and HLA-DP. The HLA class II alpha and beta genes are highly polymorphic with the exception of HLA-DR alpha gene, which is reported to be monomorphorphic. HLA class II glycoproteins are expressed as alpha/beta heterodimers on the surface of antigen presenting cells such as dendritic cells, B-cells and monocytes. Their primary function is to present foreign antigen derived peptides to CD4 positive T-cells. HLA-class II molecules also play an important role in developing and maintaining tolerance to self-antigens and in transplant rejection. HLA-DR molecules have been associated with a range of autoimmune diseases including rheumatoid arthritis, Grave’s disease and multiple sclerosis.
Antibody come fromBra30 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with REH-6 cells, a human pre-B cell leukaemia cell line.
Other descriptionEach vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
Antigen-antibody binding interactionMouse anti HLA-DR Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityThe antibody Bra30 is directed against a monomorphic determinant of human HLA Class II. The antibody recognises an epitope on the HLA-DR molecule that is dependent on the association of both heavy and lights chains of the HLA class II molecule. The antibody does not cross react with either HLA-DP or HLA-DQ molecules. The antibody has not been tested for cross reactivity with other species.
Research interestImmunology
ApplicationFlow Cytometry, Immunocytochemistry, Immunohistology (frozen), Immunoprecipitation, Western Blotting
Antibody's suited forThe Bra30 antibody is suitable for the detection of HLA Class II antigen by flow cytometry, immunocytochemistry and immunohistochemistry on frozen tissues. The antibody is also suitable for the detection of HLA class II by immunoprecipitation and western blotting. Optimal antibody dilutions for the different applications should be determined by titration.
StorageStore at 4oC, or in small aliquots at -20oC.
Relevant references1. Poláková K, Karpatová M.(1990).Study of monomorphic determinants on DR molecules of HLA class II antigens. Neoplasma. 37:239-51. 2. Lahat N, Bitterman H, Weiss-Cerem L, Rahat MA.(2011). Hypoxia increases membranal and secreted HLA-DR in endothelial cells, rendering them T-cell activators.Transpl. Int. 24:1018-26.
Protein numberUniProt: P01903
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus