Mouse anti CD8

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Main information

NameMouse anti CD8
Catalog numberMUB2055P
Price€ 290.00

More details

CategoryPrimary Antibodies
Long descriptionCD8 is a 32kDa cell surface transmembrane glycoprotein expressed by most thymocytes, a subpopulation of mature peripheral blood T-cells and natural killer (NK) cells. CD8 is expressed on the cell surface as a heterodimer, comprising two distinct disulphide linked α and β polypeptide chains, or as a CD8α homodimer. The CD8 αβ heterodimer is expressed on T-cell receptor (TCR) αβ+ cytotoxic T-lymphocytes (CTL’s), whereas CD8α homodimers are expressed on TCR γδ+ T-cells, natural killer cells and a subpopulation of TCR αβ+ T-cells. The CD8 molecule functions as a co-receptor (with the T-cell receptor) as part of the antigen-recognition complex on TCRαβ+, class I MHC-restricted T cells and plays an important role in T-cell signaling. The CD8 Ig-like domain binds to the alpha-3 domain of MHC-Class 1.
Antibody come fromRIV11 is a mouse monoclonal IgG1 antibody derived by fusion of 653 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human peripheral blood lymphocytes.
Other descriptionEach vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
Antigen-antibody binding interactionMouse anti CD8 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityThe antibody RIV11 is directed against human CD8 and recognises all CD8 positive T-cells, thymocytes and natural killer cells.
Research interestCD Marker, Immunology
ApplicationFlow Cytometry, Immunohistochemistry (Frozen), Immunocytochemistry, ELISA
Antibody's suited forThe RIV11 antibody is suitable for the detection of total CD8 by flow cytometry and ELISA. The antibody is also suitable for the detection of CD8 by immunocytochemistry and immunohistochemistry on frozen tissues. Human lymph node, spleen and peripheral blood lymphocytes are suitable for use as positive controls. Optimal antibody dilutions for the different applications should be determined by titration.
StorageStore at 4oC, or in small aliquots at -20oC.
Relevant references1. Yurchenko OV, Shlapatska LM, Skryma MR, Berdova GG, Kovalevska LM, Tarasova TA, Yurchenko MY, Kulik GI, Sidorenko SP. 2003 Immunohistochemical studies of CD150 expression in some human tumors. Exp Oncol.25:186 – 190.
Protein numberUniProt: P01732 (Human)
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus