Swine anti Mouse IgA (Fc specific)

Here you can find detailed information about selected products. Contact with us if you have nay questions to product or order proces.


Main information

NameSwine anti Mouse IgA (Fc specific)
Catalog numberSwAM/IgA(Fc)
Size1 mL
Price€ 209.00


More details

CategorySecondary Antibodies
Long descriptionThe reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in mouse serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Antibody come fromHighly purified pools of monoclonal IgA isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal swine serum. No foreign proteins added.
ClonePolyclonal
Antigen-antibody binding interactionSwine anti Mouse IgA (Fc specific) Antibody
Antibody is raised inSwine
Antibody's reacts withMouse
Antibody's reacts with these speciesThe antiSerum does not cross-react with any other component of the Mouse immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
Antibody's specificityPrecipitating polyclonal Goat antiSerum to Mouse IgA, Fc specific.
Research interestVeterinary
ApplicationImmunoprecipitation
Antibody's suited forImmunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitin titre 1:16 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
Relevant referencesno information yet
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
PropertiesImmunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
Additional isotypeIgA
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus