Mouse anti phosphotyrosine

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Main information

NameMouse anti phosphotyrosine
Catalog numberX1021
Price€ 319.00

More details

CategoryPrimary Antibodies
Long descriptionThe role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function.
Antibody come fromHybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Other descriptionProvided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide.
Antigen-antibody binding interactionMouse anti phosphotyrosine Antibody
Antibody is raised inMouse
Antibody's reacts withUbituitous
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunoprecipitation, Enzyme Immunoassay, Immunohistochemistry
Antibody's suited forApplication: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry or immunocytochemistry.
Relevant references1. Druker, B. J., Mamon, H. J. and Roberts, T. M. (1989) Oncogenes, growth factors, and signal transduction. New Eng. J. Med. 321: 1383-1391. _x000B__x000B_2. Frackelton, R. A., Jr., Ross, A. H. and Eisen, H. N. (1983) Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed and growth factor-stimulated cells. Mol. Cell. Biol. 3: 1343-1352. _x000B__x000B_3.Glenney, J. R., Jr., Zokas, L. and Kamps, M. P. (1988) Monoclonal antibodies to phosphotyrosine. J. Imm. Meth. 109: 277-285. _x000B__x000B_4.Wang, J. Y. J. (1988) Antibodies for phosphotyrosine: Analytical and preparative tool for tyrosyl- phosphorylated proteins. Anal. Biochem. 172: 1-7. _x000B__x000B_5. Sikkema, A.H., et al. Kinome Profiling in Pediatric Brain Tumors as a New Approach for Target Discovery. Cancer Res., 69, 5987-5995 (2009).
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus