Mouse anti TCF-4

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Main information

NameMouse anti TCF-4
Catalog numberX1070M
Size100 μg
Price€ 345.00


More details

CategoryPrimary Antibodies
Long descriptionTCF 4 (T cell factor 4) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 4 generates transcriptionally active complexes with catenin in colon carcinoma cells. Expression of TCF 4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF 4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.
Antibody come fromHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein.
Other descriptionProvided as 0.2µm sterile filtered solution in 0.7 M tris-glycine, pH 7.4, 0.105M NaCl, 30% glycerol with 0.035% sodium azide. Protein A/G Chromatography
Clone6H5.3
Antigen-antibody binding interactionMouse anti TCF-4 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman, Mouse
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunohistochemistry, Immunoprecipitation
Antibody's suited forDetects TCF-4 by Western blot at 1-4 µg/ml. Detects a 66 kDa band in Cos-1 cells transfected with human TCF-4. Can also be used for immunohistochemistry of 4% paraformaldehyde fixed tissues, immunoprecipitation and supershift assays. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Barker, N., et al. Resticted high level expression of TCF-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 1999, 154, 29-35 _x000B__x000B_2. Ishitani, M.L., et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999, 399, 798-802_x000B__x000B_3. Korinek, V., et al. Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nature Genetics 1998, 19, 379-383
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus