Mouse anti TCF-1

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Main information

NameMouse anti TCF-1
Catalog numberX1072M
Size100 μg
Price€ 474.00

More details

CategoryPrimary Antibodies
Long descriptionTCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).
Antibody come fromHybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 102-269 from the human TCF1A splice variant protein fused to maltose binding protein (MBP)
Other descriptionProvided as 0.2µm sterile filtered solution in 0.1 M tris-glycine, pH 7.4, 0.150M NaCl, 30 % glycerol with 0.08% sodium azide. Protein A/G Chromatography
Antigen-antibody binding interactionMouse anti TCF-1 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunohistochemistry (Frozen Sections)
Antibody's suited forDetects multiple human TCF-1 isotypes by Western blot at 0.5 to 2 µg/ml. Detects bands at 26, 32, 38, 42 and 48 kDa in Jurkat cell lysates. Optimal concentration should be evaluated by serial dilutions.
Relevant references1. Staal, F.J., et al. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int. Immunol. 1999, 11, 317-323_x000B__x000B_2. Mayer, K., et al. Ectopic activation of lymphoid high mobility group-box transcription factor TCF-1 and overexpression in colorectal cancer cells. Int. J. Cancer 1997, 72, 625-630_x000B__x000B_3. Castrop, K., et al. The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-linkage lymphocytes. Blood 1995, 86, 3050-3059_x000B__x000B_4. Van de Wetering, M, et al. 'Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties.' Mol. Cell. Biol., 16: 745-52 (1996)._x000B__x000B_Product Specific References:_x000B__x000B_1. Lin, C-W, et al. 'CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.' Blood, 2005, 106, 3567-3574.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus