Mouse anti LEF-1 all isoforms

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Main information

NameMouse anti LEF-1 all isoforms
Catalog numberX1076M
Size100 μg
Price€ 319.00

More details

CategoryPrimary Antibodies
Long descriptionLymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.
Antibody come fromRecombinant full length LEF-1 protein
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Antigen-antibody binding interactionMouse anti LEF-1 all isoforms Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot
Antibody's suited forAntibody detects all LEF-1 isoforms by Western blot. Does not cross-react with TCF-1 or TCF-4. Optimal concentration should be evaluated by serial dilutions.
Relevant references1. Riese, J., et al., LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic. Cell 1997, 88, 777-787_x000B__x000B_2. Giese, K., et al. Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1. Proc. Natl. Acad. Sci. USA 1997, 94, 12845-12850_x000B__x000B_3. Behrens, J., et al. Functional interaction of beta-catenin with the transcription factor LEF-1. Nature 1996, 382, 638-642_x000B__x000B_4. Giese, K. and Grosschedl, R. LEF-1 contains an activation domain that stimulates transcription only in a specific context of factor-binding sites. EMBO J. 1993, 12, 4667-4676
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus