Mouse anti PIG3 (p53 inducible gene 3)

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Main information

NameMouse anti PIG3 (p53 inducible gene 3)
Catalog numberX1156M
Size100 μg
Price€ 304.00


More details

CategoryPrimary Antibodies
Long descriptionp53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.
Antibody come fromHybridoma produced by the fusion of splenocytes from mice immunized with recombinant human PIG-3 protein.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone10A2
Antigen-antibody binding interactionMouse anti PIG3 (p53 inducible gene 3) Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot
Antibody's suited forAntibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Flatt, P.M., et al., p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Cancer Lett. 2000, 156, 63-73_x000B__x000B_2. Venot, C., et al., The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. EMBO J. 1998, 17, 4668-4679_x000B__x000B_3. el-Deiry, W.S., Regulation of p53 downstream genes. Semin. Cancer Biol. 1998, 8, 345-357
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus