Mouse anti p21 WAF-1

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Main information

NameMouse anti p21 WAF-1
Catalog numberX1158M
Size100 μg
Price€ 304.00

More details

CategoryPrimary Antibodies
Long descriptionp21, also known as wild-type p53-activated factor (WAF-1) or Cdk-interacting protein (CIP1), expression is predominantly induced by p53. This expression is usually in response to DNA damage caused by cytotoxic agents. The expression of p21 interrupts the cell cycle and prevents the replication of the cell containing genomic errors. p21 inhibits Cdk2, Cdk3, Cdk4 and Cdk6 to stop the cell cycle. Contained in the human p21 is a highly conserved cystine-rich region (amino acids 21-60) with a potential zinc finger domain as well as a second conserved region (amino acids 130-164) which contains several putative nuclear localization signals. p21 can also interact with proliferating cell nuclear antigen (PCNA), Gadd45, Cdk2 and cyclins which suggests it may coordinate DNA repair and replication in damaged cells.
Antibody come fromHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human p21 protein and mouse myeloma SP2 cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Antigen-antibody binding interactionMouse anti p21 WAF-1 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, ELISA
Antibody's suited forDetects p21 protein by Western blot at 1 to 10 µg/ml. Detects a 21 kDa protein in MCF7 cells. Can be used with either frozen or paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: ELISA
Relevant references1. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878 _x000B__x000B_2. El-Deiry, W.S., et al. WAF1, a potential mediator of p53 tumor suppression. Cell 1993, 75, 817-825 _x000B__x000B_3. Xiong, Y., et al. p21 is a universal inhibitor of cyclin kinases. Nature (Lond) 1993, 366, 701-704 4. Zhang, H., et al. Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol. Biol. Cell 1993, _x000B__x000B_4, 897-906 _x000B__x000B_5. Kovalic, J., et al. Int. J. Oncol. 1996, 9 (suppl.), 835 _x000B__x000B_6. Shen, J., et al. ÔIdentification and Validation of Differences in Protein Levels in Normal, Premalignant, and Malignant Lung Cells and Tissues Using High-Throughput Western Array and Immunohistochemistry.Õ Cancer Res., 66, 11194-11206 (2006).
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus