Mouse anti 6x-His Tag

Here you can find detailed information about selected products. Contact with us if you have nay questions to product or order proces.


Main information

NameMouse anti 6x-His Tag
Catalog numberX1181M
Size100 μg
Price€ 304.00


More details

CategoryPrimary Antibodies
Long description6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.
Antibody come fromHybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Clone2C5.1
Antigen-antibody binding interactionMouse anti 6x-His Tag Antibody
Antibody is raised inMouse
Antibody's reacts withN/A
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot
Antibody's suited forDetects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Zentgraf, H., et al. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 1995, 23, 3347-3348_x000B__x000B_2. Sisk, W.P., et al. High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J. Virol. 1994, 68, 766-775_x000B__x000B_3. Hosfield, T. and Lu, Q. S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning. Biotechniques 1999, 27, 50-60
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Conjugationhistidine
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus