Mouse anti Keratin 13 & 10

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Main information

NameMouse anti Keratin 13 & 10
Catalog numberX1252M
Size100 μg
Price€ 304.00


More details

CategoryPrimary Antibodies
Long descriptionCytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification._x000B__x000B_This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. These proteins correspond to CK numbers 13 and 10, respectively, according to Moll's classification1. Using formalin-fixed, paraffin-embedded tissue sections, this antibody detects only the CK13. With frozen sections, this antibody serves as a differentation-related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Antibody come fromHybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneDE-K13
Antigen-antibody binding interactionMouse anti Keratin 13 & 10 Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationWestern Blot, Immunohistochemistry (Frozen & Paraffin Sections)
Antibody's suited forDetects proenzyme form of human Caspase-7 by Western blot. Optimal concentration should be evaluated by serial dilutions.
Storage-20ºC
Relevant references1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16_x000B__x000B_2. Chandler, J.M., et al. Different subcellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver. J. Bio. Chem. 1998, 273, 10815-10818_x000B__x000B_3. Behrensdorf, H.A., et al. The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7. FEBS Lett. 2000, 466, 143-147
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus